7aad (Miltenyi Biotec)
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Structured Review
Miltenyi Biotec
7aad
7aad, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/7aad/7-AAD+Staining+Solution/us12698315-681-21-22
Average 97 stars, based on 81 article reviews
7aad, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/7aad/7-AAD+Staining+Solution/us12698315-681-21-22
Average 97 stars, based on 81 article reviews
7aad - by Bioz Stars,
2026-09
97/100 stars
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Incubation:Article Title: Method for producing t cells modified by chimeric antigen receptor Article Snippet: .. SA-PE, CD3-APC, CD4-PE-Cy7, CD8-VioBlue and Expressing:Article Title: Method for producing t cells modified by chimeric antigen receptor Article Snippet: .. SA-PE, CD3-APC, CD4-PE-Cy7, CD8-VioBlue and Flow Cytometry:Article Title: Method for producing t cells modified by chimeric antigen receptor Article Snippet: .. SA-PE, CD3-APC, CD4-PE-Cy7, CD8-VioBlue and Staining:Article Title: Polyclonal expansion of functional tumor-reactive lymphocytes infiltrating glioblastoma for personalized cell therapy Article Snippet: .. All samples were stained with Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction Article Snippet: .. PBMCs isolated from PMMN cohort patients through Ficoll separation were first stained for surface markers for 30 minutes at 4°C in blocking solution (PBS, 5% FBS, 1% BSA, 2 mM EDTA) containing Brilliant stain buffer plus (BD Biosciences, Franklin Lakes, NJ) and fluorochrome-labeled monoclonal antibodies against CD45 (clone 2D1; RRID:AB_2814897; BD Biosciences); CD19 (clone SJ25C1; RRID:AB_1645728; BD Biosciences); CD3 (clone SK7; RRID:AB_2833003; BD Biosciences); CD4 (clone RPA-T4; RRID:AB_2744420; BD Biosciences); CD8 (clone SK1; RRID:AB_2868801; BD Biosciences); CD25 (clone 2A3; RRID:AB_2868687; BD Biosciences); and Article Title: Method for transduction of t cells in the presence of malignant cells Article Snippet: .. After binding for 1 h at 4° C., excess LV was removed by one washing step and staining was performed with Article Title: γδ T Cells Target and Ablate Senescent Cells in Aging and Alleviate Pulmonary Fibrosis Article Snippet: Cells were washed with 1 ml of ice-cold PBS and resuspended in 200 μl of ice-cold PBS and analyzed on the MACSQuant10, Miltenyi Biotec flow cytometer. .. Cell viability was determined by Software:Article Title: Polyclonal expansion of functional tumor-reactive lymphocytes infiltrating glioblastoma for personalized cell therapy Article Snippet: .. All samples were stained with Isolation:Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction Article Snippet: .. PBMCs isolated from PMMN cohort patients through Ficoll separation were first stained for surface markers for 30 minutes at 4°C in blocking solution (PBS, 5% FBS, 1% BSA, 2 mM EDTA) containing Brilliant stain buffer plus (BD Biosciences, Franklin Lakes, NJ) and fluorochrome-labeled monoclonal antibodies against CD45 (clone 2D1; RRID:AB_2814897; BD Biosciences); CD19 (clone SJ25C1; RRID:AB_1645728; BD Biosciences); CD3 (clone SK7; RRID:AB_2833003; BD Biosciences); CD4 (clone RPA-T4; RRID:AB_2744420; BD Biosciences); CD8 (clone SK1; RRID:AB_2868801; BD Biosciences); CD25 (clone 2A3; RRID:AB_2868687; BD Biosciences); and Blocking Assay:Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction Article Snippet: .. PBMCs isolated from PMMN cohort patients through Ficoll separation were first stained for surface markers for 30 minutes at 4°C in blocking solution (PBS, 5% FBS, 1% BSA, 2 mM EDTA) containing Brilliant stain buffer plus (BD Biosciences, Franklin Lakes, NJ) and fluorochrome-labeled monoclonal antibodies against CD45 (clone 2D1; RRID:AB_2814897; BD Biosciences); CD19 (clone SJ25C1; RRID:AB_1645728; BD Biosciences); CD3 (clone SK7; RRID:AB_2833003; BD Biosciences); CD4 (clone RPA-T4; RRID:AB_2744420; BD Biosciences); CD8 (clone SK1; RRID:AB_2868801; BD Biosciences); CD25 (clone 2A3; RRID:AB_2868687; BD Biosciences); and Bioprocessing:Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction Article Snippet: .. PBMCs isolated from PMMN cohort patients through Ficoll separation were first stained for surface markers for 30 minutes at 4°C in blocking solution (PBS, 5% FBS, 1% BSA, 2 mM EDTA) containing Brilliant stain buffer plus (BD Biosciences, Franklin Lakes, NJ) and fluorochrome-labeled monoclonal antibodies against CD45 (clone 2D1; RRID:AB_2814897; BD Biosciences); CD19 (clone SJ25C1; RRID:AB_1645728; BD Biosciences); CD3 (clone SK7; RRID:AB_2833003; BD Biosciences); CD4 (clone RPA-T4; RRID:AB_2744420; BD Biosciences); CD8 (clone SK1; RRID:AB_2868801; BD Biosciences); CD25 (clone 2A3; RRID:AB_2868687; BD Biosciences); and Recombinase Polymerase Amplification:Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction Article Snippet: .. PBMCs isolated from PMMN cohort patients through Ficoll separation were first stained for surface markers for 30 minutes at 4°C in blocking solution (PBS, 5% FBS, 1% BSA, 2 mM EDTA) containing Brilliant stain buffer plus (BD Biosciences, Franklin Lakes, NJ) and fluorochrome-labeled monoclonal antibodies against CD45 (clone 2D1; RRID:AB_2814897; BD Biosciences); CD19 (clone SJ25C1; RRID:AB_1645728; BD Biosciences); CD3 (clone SK7; RRID:AB_2833003; BD Biosciences); CD4 (clone RPA-T4; RRID:AB_2744420; BD Biosciences); CD8 (clone SK1; RRID:AB_2868801; BD Biosciences); CD25 (clone 2A3; RRID:AB_2868687; BD Biosciences); and Binding Assay:Article Title: Method for transduction of t cells in the presence of malignant cells Article Snippet: .. After binding for 1 h at 4° C., excess LV was removed by one washing step and staining was performed with |